BACTERIAS DEGRADADORAS DE HIDROCARBUROS AISLADAS EN SUELOS CONTAMINADOS DE PETROLEO Aislar cepas bacterianas y. Aislamiento de bacterias potencialmente degradadoras de petróleo en hábitats de ecosistemas costeros en la Bahía de Cartagena, Colombia. Download Citation on ResearchGate | SELECCIÓN DE BACTERIAS CON CAPACIDAD DEGRADADORA DE HIDROCARBUROS Estudio y selección de bacterias aerobias degradadoras de hidrocarburos del petróleo aisladas de costas.

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Microbiol Mol Biol Rev. Each strain was assayed in triplicate. Salt tolerance was estimated by seeding the test strains in marine bacteria isolation medium where seawater was replaced by distilled water and sodium chloride NaCl concentration was set at 0, 0.

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The obtained bacterial suspensions constituted the inocula. Morphological descriptions were based on bacterial shape, motility and pigmentation.

Field evaluations of marine oil spill bioremediation. F10S1 and Bacillus sp.

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In both protocols the marine bacteria isolation plates were periodically examined after 24 h of growth under a stereoscope, streaking onto bacferias plates degrafadoras the colonies appearing during the seven day incubation period. Biodegradation of crude oil contaminating marine shorelines and freshwater wetlands.

Usually found at low concentrations in non-contaminated areas, their populations bloom in chronically contaminated environments [1]. Impact of irradiation and polycyclic aromatic hydrocarbon spiking on microbial populations in marine sediment for future aging and biodegradability studies.

Lastly, the genera Kurthia and Azotobacter were represented each by a single strain. Appl Environ Microbiol ;64 Varadero crude is higher in asphaltenes, whereas the main constituents of Pina crude are saturated hydrocarbons with chain lengths smaller than 18 carbon atoms.

Biodegradability of Venezuela crude oils. Peetroleo addition, other authors have shown that the biotransformation of asphaltenes and resins leads to the accumulation of simpler saturated and aromatic derivates, increasing the concentration of these fractions [53, 54].


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Monocyclic and polycyclic aromatic hydrocarbon fractions were analyzed by FTIR spectroscopy, as described fegradadoras Materials and Methods. Culturing these strains in the presence of hydrocarbons Mesa 30 crude favored the expression of enzyme systems involved in their degradation and the preferential isolation of hydrocarbon-tolerant clones. The obtained isolates were then subjected to selection in Bushnell-Haas medium supplemented with a heavy crude oil, selecting three strains able to degrade this hydrocarbon mixture within a period of seven days.

Trials of bioremediation on a beach affected by the heavy oil spill of gacterias Prestige.

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Two of the isolated strains belonged to the Pseudomonas genus. The present study has demonstrated that the bacterial strains selected are able to degrxdadoras hydrocarbons as sole carbon source when grown in pure cultures.

Pure cultures of these strains were further used in crude oil biodegradability assays. ABSTRACT The isolation of aerobic marine bacteria able to degrade hydrocarbons represents a promising alternative for the decontamination of oceanic and coastal environments.

Based on our results, however, together with the existing literature, it is possible to make some inferences. Bioremediation of petroleum-and creosote-contaminated soils: Bioremediation potential of terrestrial fuel spills. They were taxonomically identified as members of the Bacillus, Alcaligenes, Pseudomonas, Acinetobacter, Marinomonas, Kurthia and Azotobacter genera. Taxonomy of aerobic marine eubacteria.

Comparative physiology of phenanthrene degradation by two dissimilar pseudomonads isolated from a creosote-contaminated soil. It has been shown that strain WW1 of Alcaligenes denitrificans can degrade four-ring polyaromatic hydrocarbons [30]. Five replicates were used per sample. Hydrocarbons are lipophilic compounds that inhibit growth when present at high concentrations [45], inducing the bacterial stress response and a series bacrerias changes at the membrane, enzyme and protein levels [44, 46].

The degradation potential exhibited by these new isolates warrants further studies on their possible application to decontaminate coastal environments affected by oil spills.


Enhanced biodegradation of Casablanca crude oil by a microbial consortium in presence of a rhamnolipid produced by Pseudomonas aeruginosa AT An analysis of these results leads us to suppose that linear chains up to 30 carbon atoms long and some low molecular weight aromatic compounds were degraded during the first 10 to 15 days. Publications reporting the presence of this genus petorleo hydrocarbon-contaminated ecosystems and describing its hydrocarbon-degrading abilities have appeared in the literature from the early nineties [17], although its numbers have increased as of late [19, 24, 25, 31, 32].

Another two strains belonged to the petrolwo Acinetobacter and Marinomonasrespectively. Strain F10S1 lowered the concentration of all fractions in comparison with the control. Microbial Transformation and degradation of toxic organic chemicals. Since hydrocarbon determinations were performed solely at the end of the study day 45no data are available to evaluate the biodegradation process in earlier time points. Regardless, Joseph et al.

This decrease in isoprenoids is a telltale sign of effective biodegradation; whereas strain F1FLC exhibits values above those of the abiotic control. Most research on bioremediation technology has focused therefore on aerobic heterotrophic bacteria, due not only to the taxonomic diversity of hydrocarbon-degrading representatives from this group, but to their ability to use xenobiotic compounds as carbon source in pure cultures [3].

SPE International conference on health, safety, and environment in oil and gas exploration and production, JuneCaracas, Venezuela. Three replicates were seeded per strain.